近藤 昭彦 サッカー - 少年にわいせつ行為…元サッカー監督に懲役８年― スポニチ Sponichi Annex サッカー

This is the first report of overcoming CCR by disrupting α-tubulin gene in T. Comparative profiling analysis of central metabolites in Euglena gracilis under various cultivation conditions. Construction of pichia pastoris cell-surface display system using Flo1p anchor system. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions. Immobilized recombinant Aspergillus oryzae expressing heterologous lipase: an efficient whole-cell biocatalyst for enantioselective transesterification in non-aqueous Medium. Journal of Forest research, 3, 111-117. To explore terminator sequences available to tune protein expression levels in P. Review 2014 Genetic engineering of bio-nanoparticles for drug delivery: a review. Paramylon, an insoluble dietary fibre that accumulates in E. Bioconversion of lignocellulose-derived sugars to ethanol by engineeredSaccharomyces cerevisiae. Hiroaki Abe, Changyuan Tang, Akihiko Kondoh 2014 : Effect of Urban Aquifer Exploitation on Subsurface Temperature and Water Quality. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase Target-ACEmax showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. Production of ethanol as a major byproduct was completely inhibited, and the limited translocation of pyruvate into the mitochondria shifted the metabolism from respiration for energy conversion to the effective production of lactate or 2,3-butandiole, even under aerobic conditions. Production of biodiesel fuel from soybean oil catalyzed by fungus whole-cell biocatalysts in ionic liquids. Conclusion Trichoderma reesei α-tubulin is involved in the transcription of cellulase and hemicellulase genes, as well as in CCR. Market-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae. Hydrological Research Letters, 14 1 , 56—61. Jianyao Chen, Changyuan Tang, Yasuo Sakura, Akihiko Kondoh, Jingjie Yu, Jun Shimada and Tadashi Tanaka 2004 :Spatial geochemical and isotopic characteristics associated with groundwater flow in the North China Plain. Direct Production of Cadaverine from Soluble Starch Using Corynebacterium glutamicum Coexpressing α-Amylase and Lysine Decarboxylase. Groundwater and Subsurface Environment. Construction of ethanol-tolerant yeast strains with combinatorial library-selected peptides. The disruption of α-tubulin is a promising approach for creating next-generation enzyme-producing strains of T. Advances in Space Research, 26 7 , 1173-1176. Dispersion of coagulated particles by contractile flow to orifice. Improvement of isoflavone aglycones production using β-glucosidase secretory produced in recombinant Aspergillus oryzae. Efficient fermentation of xylose to ethanol at high formic acid concentrations by metabolically engineered Saccharomyces cerevisiae. Double mutation of the PDC1 and ADH1 genes improves lactate production in the yeast Saccharomyces cerevisiae expressing the bovine lactate dehydrogenase gene. Improvement of ethanol productivity during xylose and glucose co-fermentation by xylose-assimilating S. Review Bioenergy: Sustainable fuels from biomass by yeast and fungal whole-cell biocatalysts. The product portfolio of the microbe is continuously expanding. Improved homo L-lactic acid fermentation from xylose by abolishment of the phosphoketolase pathway and enhancement of the pentose phosphate pathway in genetically modified xylose-assimilating lactococcus lactis. Fadong Li, Xinfabg Song, Changyuan Tang, Akihiko Kondoh, Wanjun Zhang 2008 : Stable isotopic charavterisation of precipitation, soil water and groundwater in Tanhang Mountain, north China. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Characterization of fractionated polyclonal antibodies for immunoaffinity chromatography. Preparation of yeast strains displaying IgG binding domain ZZ and enhanced green fluorescent protein for novel antigen detection systems. However, microorganisms cannot directly produce 1,3-butadiene using glucose as a renewable carbon source via biological processes. Glutamate production from β-glucan using endoglucanase-secreting Corynebacterium glutamicum. Characterization of the immunointeraction and its application to bioaffinity separation. Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media. Selection of DNA aptamers using atomic force microscopy. Long anchor using Flo1 protein enhances reactivity of cell surface-displayed glucoamylase to polymer substrates. Novel strategy for yeast construction using δ-integration and cell fusion to efficiently produce ethanol from raw starch. Nguyen Cung Que Truong, Hong Quan Nguyen and Akihiko Kondoh 2018 : Land Use and Land Cover Changes and Their Effect on the Flow Regime in the Upstream Dong Nai River Basin, Vietnam. From transcriptome analysis, the Δ tubB strain exhibited upregulation of both cellulase and hemicellulase genes including some that were not originally induced by cellulose. Dimensionality reduction for metabolome data using PCA, PLS, OPLS, and RFDA with diffenential penalties to latent variables. Development of an Aspergillus oryzae whole-cell biocatalyst coexpressing triglyceride and partial glyceride lipases for biodiesel production. Additionally, in pure cultures, B. Yuki Tosaki, Norio Tase, Akihiko Kondoh, Kimikazu Sasa, Tsutomu Takahashi, Yasuo Nagashima 2011 : Distribution of 36Cl in the Yoro River Basin, central Japan and its relation to regional groundwater flow system. Dilinur Aji, Akihiko Kondoh, Changyuan Tang 2008 :Analysis of hydrological changes of lakes and rivers in XinJiang using GIS techniques and remote sensing data. Development and application of thermo-sensitive magnetic immunomicrospheres for antibody purification. Rapid and efficient selection of yeast displaying a target protein using thermo-responsive magnetic nanoparticles. Control of signalling properties of human somatostatin receptor subtype-5 by additional signal sequences on its amino-terminus in yeast. Use of mono-and diacylglycerol lipase as immobilized fungal whole cells to convert residual partial glycerides enzymatically into fatty acid methyl esters. Comparison of two forms of catalytic antibody displayed on yeast-cell surface. Evaluation of the biodegradability of polyurethane and its derivatives by using lipase-displaying arming yeast. Characterization of bio-nanocapsule as a transfer vector targeting human hepatocyte carcinoma by disulfide linkage modification. Development of a novel aptamer-based sensing system using atomic force microscopy. Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction. Review 2012 Consolidated bioprocessing and simultaneous saccharification and fermentation of lignocellulose to ethanol with thermotolerant yeast strains. Metabolomic and transcriptomic analyses showed an emphasis on glycolysis and a repressed TCA cycle. Enhancement of activity of lipase-displaying yeast cells and their application to optical resolution of RS -1-benzyloxy-3-chloro-2-propyl monosuccinate. In addition, YM administration increased the production of acetate, propionate, and total short-chain fatty acids. The ratio was correlated with the pgi transcription level and the enzyme activity. Akihiko Kondoh, Agung Budi Harto, Runtunuwu Eleonora, and Toshiharu Kojiri 2004 : Hydrological regions in monsoon Asia. KOR1 was subsequently characterized with extensive mutation of the ISA1 gene encoding a starch debranching enzyme DBE. An in vitro microbiota model was established using fecal inocula collected from nine healthy volunteers, and each model was found to retain operational taxonomic units similar to the ones in the original human fecal samples. Richa Bhattarai, Haireti Alifu, Aikebaier Maitiniyazi, Akihiko Kondoh 2017 : Detection of Land Subsidence in Kathmandu Valley, Nepal, Using D-InSAR Technique, Land, 6, 39, doi:10. Use of a substrate as an encapsulated marker for liposome immunoassay. Hydrogeology Journal, 10, 587-599. Protein substrates and heat shock reduce the DNA-binding ability of esherichia coli lon protease. Effect of cereal extracts and cereal fiber on viability of Lactobacillus plantarum under gastrointestinal tract conditions. D-lactic acid production from cellooligosaccharides and beta-glucan using L-LDH gene-deficient and endoglucanase-secreting Lactobacillus plantarum. Wei Yang, Hideki Kobayashi, Kenlo Nishida Nasahara, Rikie Suzuki, Akihiko Kondoh 2017 : Quantitative Evaluation of Bitterlich Sampling for Estimating Basal Area in Sparse Boreal Forests and Dense Tropical Forests, , 7, 143-156. Engineered bio-nanocapsules, the selective vector for drug delivery system. Consequently, the lipid-rich mutant Chlamydomonas sp. Variation in biomass properties among rice diverse cultivars. Construction of whole-cell biocatalyst for xylan degradation through cell-surface xylanase display in Saccharomyces cerevisiae. Over-expression system for secretoryphospholipase D by streptomyceslividans. Proceedings of the 9TH Scientific Meeting of Indonesian Students, Shizuoka, Japan, pp. Int'l Workshop on Global Change and Terrestrial Environment in Monsoon Asia, 95-98. Preparation and comparative characterization of immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase for enzymatic biodiesel production. Effects of adsorption conditions on kinetics of protein adsorption and conformational changes at ultrafine silica particles. This study constructed a novel artificial metabolic pathway for 1,3-butadiene production from glucose in Escherichia coli by combining the cis,cis-muconic acid ccMA -producing pathway and tailored ferulic acid decarboxylase mutants. Review 2012 Enzyme-mediated methodologies for protein modification and bioconjugate synthesis. Transesterification of phosphatidylcholine in sn-1 position through direct use of lipase-producing Rhizopus oryzae cells as whole-cell biocatalyst. Conclusively, our results indicate that the redirection of pyruvate metabolism is a useful strategy in the metabolic engineering of aerobic microorganisms. Using dissolved oxygen—stat fed-batch fermentation in a 1-L jar fermenter, we could produce 2. The non-conventional yeast Pichia pastoris syn. Ethanolysis of rapeseed oil to produce biodiesel fuel catalyzed by Fusarium heterosporum lipase-expressing fungus immobilized whole-cell biocatalysts. Phospholipase D from Streptoverticillium cinnamoneum: protein engineering and application for phospholipid production. Quantitative real-time PCR analysis revealed that YM administration significantly increased the relative abundance of Bacteroides ovatus and B. System Metabolic Engineering, Chapter 10, Design of superior cell factories for a sustainable biorefinery by synthetic bioengineering. In this study, we show the effects of YM on human colonic microbiota and its metabolites using an in vitro human faeces fermentation system. Direct ethanol production from cassava pulp using a surface-engineered yeast strain co-displaying two amylases, two cellulases, and β-glucosidase. This study aims to identify promising targets for metabolic engineering of improved lipid accumulation under outdoor conditions. JSC4 through metabolic profiling and an innovative salinity-gradient strategy. Indonesian Journal of Agricultural Science, 9 11 , 7-18. Improvement of productivity of active horseradish peroxidase in esherichia coli by coexpression of Dsb proteins. Journal of the Japan Society of Photogrammetry and Remote Sensing, 39 4 , 64-73. Direct ethanol production from cellulosic materials at high temperature using the thermotolerant yeast Kluyveromyces marxianus displaying cellulolytic enzymes. Purification, characterization, and sequence determination of phospholipase D secreted by streptoverticillum cinnamoneum. Akihiko Kondoh and Changyuan Tang 1996 : Link GIS to 3D groundwater model. Conformational changes in protein molecules upon adsorption on ultrafine particles. On Hydrology, Water Resources and Environment Development, and Management in Southeast Asia and the Pacific. Review Protein-protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling. Adsorption of model proteins with wide variation in molecular properties on colloidal particles. Preparation of high-activity whole cell biocatalysts by permeabilization of recombinant yeasts with alcohol. Over-production of various secretory-form proteins in Streptomyces lividans. Efficient and direct glutathione production from raw starch using Saccharomyces cervisiae. Yongqiang Zhang, Changming Liu, Qiang Yu, Yanjun Shen, Eloise Kendy, Akihiko Kondoh, Changyuan Tang and Hongyong Sun 2004 : Energy fluxes and the Priestley-Taylor parameter over winter wheat and maize in the North China Plain, Hydrological Processes, 18, 2235-2246. Production of glucoamylase by passively immobilized cells of a flocculent yeast, Saccharomyces diastaticus. Identification of novel membrane-bound phospholipase D from Streptoverticillium cinnamoneum, possessing only hydrolytic activity. Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Yi-Zhang Zhang, Xian-Fang Song, Akihiko Kondoh, Jun Xia, Chang-Yuan Tang 2011 : Behavior, mass inventories and modeling evaluation of xenobiotic endocrine-disrupting chemicals along an urban receiving wastewater river in Henan Province, China. Production of bionanocapsules in immobilized insect cell culture using porous biomass support particles. Although the dry mycelial weights of the deletion mutants were reduced compared with those of wild type, the titer and yields of the target products were drastically increased. Environmental Research Center Papers, No. Immobilization of Protein on monodispersed colloidal silica with poly ethylene glycol spacer and application of the composites to immunological agglutination tests. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato Solanum lycopersicum L. Overproduction and purification of lon protease from esherichia coli using maltose binding protein MBP fusion system. Effect of methanol and water contents on production of biodiesel fuel from plant oil catalyzed by various lipases in a solvent-free system. Ethanol fermentation from lignocellulosic hydrolysate by a recombinant xylose- and cellooligosaccharide-assimilationg yeast strain. Overproduction of Geranylgeraniol by Metabolically Engineered Saccharomyces cerevisiae. A high-level expression vector containing selectable marker for continuous production of recombinant protein in insect cells. Development of recombinant Aspergillus oryzae whole-cell biocatalyst expressing lipase-encoding gene from candida antarctica. Direct ethanol production from barley β-glucan by sake yeast displaying Aspergillus oryzae β-glucosidase and endoglucanase. Gγ recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions. Advances in Space Research, 22 5 , 645-654. Capabilities of polymer-modified monodisperse colloidal silica particles as biomaterial carrier. Repeated batch fermentation from raw starch using a maltose transporter and amylase expressing diploid yeast strain. Operation conditions of enzyme refolding by chaperonin and recycle system using ultrafiltration. Int'l Workshop on Global Change and Terrestrial Environment in Monsoon Asia, 170-171. Construction of an Aspergillus oryzae cell-surface display system using a putative GPI-anchored protein. We found that changing dissolved oxygen and controlling pH were important factors for 1,3-butadiene production. Review Protein-protein interactions and selection introduction. Fatty acid production from butter using novel cutinase-displaying yeast. In pure cultures, DKT significantly promoted the growth of Bifidobacterium adolescentis, but not that of Fusobacterium nucleatum or Escherichia coli. Advances in Geosciences, 4, 11-16. Review Biotechnological production of enantiomeric pure lactic acid from renewable resources: recent achievements, perspectives, and limits. Lactic fermentation of cellobiose by a yeast strain displaying β-glucosidase on the cell surface. Phospholipase D production using immobilized cells of streptoverticillium cinnamoneum. Methods in molecular biology, Vol. Review Gene copy number and polyploidy on products formation in yeast. Xylose isomerase from polycentric fungus Orpinomyces: gene sequencing, cloning, and expression in Saccharomyces cerevisiae for bioconversion of xylose to ethanol. DKT was added to the in vitro microbiota model culture at a concentration of 0. Process engineering and optimization of glycerol separation in a packed-bed reactor for enzymatic biodiesel production. Efficient production of L- + -lactic acid from raw starch by Streptococcus bovis 148. PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae. The split ratio was varied by exchanging the promoter of a gene encoding glucose 6-phosphate isomerase PGI. The Future of Cranes and Wetlands, 72-85. Hydrological Processes, 18, 3147-3158. Improvement of transphosphatidylation reaction model of phospholipase D from Streptoverticillium cinnamoneum. Efficient generation of recessive traits in diploid sake yeast by targeted gene disruption and loss of heterozygosity. Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch. Application of magnetic nanoparticles in full-automated chemiluminescent enzyme immunoassay. Rot1p of Saccharomyces cerevisiae is a putative membrane protein required for normal levels of the cell wall β-1, 6-glucan. Overproduction and purification of SulA fusion protein in esherichia coli and its degradation by lon protease in vitro. Secretory production system of bionanocapsules using a stably transfected insect cell line. Ahmad AL-HANBALI, Hussam AL-BILBISI, Akihiko, KONDOH 2006 : Monitoring the Dead Sea Area Changes Using Remote Sensing and GIS. Proceedings of the CEReS International Symposium on remote Sensing of the Atmosphere and validation of Satellite Data, CEReS, Chiba, Japan, pp. Successful tuning of protein expression levels was shown not only for the reporter gene used to define the catalog but also using betaxanthin production as an example application in pathway flux regulation. Improvement of proteolytic resistance of immunoadsorbents by chemical modification with polyethylene glycol. The competitor-introduced Gγrecruitment system, a new approach for screening affinity-enhanced proteins. In contrast, concentrations of alanine, leucine, and valine were increased with an increase of the split ratio into glycolysis. Lipase localization in rhizopus oryzae cells immobilized within biomass support particles for use as whole-cell biocatalysts in biodiesel-fuel production. Komagataella phaffii has frequently been used as a platform for metabolic engineering but knowledge regarding P. Altogether, we found that the terminators have a tunable range of 17-fold. PCC 6803, probed by time-resolved fluorescence spectroscopy. Moreover, metabolically engineered strains of C. The two edited lines showed significant differences in carotenoid accumulation. Bioconversions in aqueous two-phase system using enzymes immobilized on ultrafine silica particles. Characterization of yeast cell surface displayed Aspergillus oryzae β-glucosidase I with high hydrolytic activity on soy bean isoflavone. Metabolic pathway engineering based on metabolomics confers acetic and formic acid tolerance to a recombinant xylose-fermenting strain of Saccharomyces cerevisiae. Pretreatment of immobilized Candida antarctica lipase for biodesel fuel production from plant oil. Preparation of a whole-cell biocatalyst of mutated candida antaretica lipase B mCALB by a yeast molecular display system and its practical properties. Atsushi Higuchi, Akihiko Kondoh, Shinkichi Kishi and Teruki Fukuzono 1996 : Relatioship between hydrological properties and multi-temporal AVHRR data in 1990, Huaihe river basin, China. Sharma, Ryutaro Tateishi, Akihiko Kondoh, Bayaer Wuliangha, and Gegen Tana 2017 : A Detailed and High-Resolution Land Use and Land Cover Change Analysis over the Past 16 Years in the Horqin Sandy Land, Inner Mongolia. Application of remote sensing and GIS for anthropogenic vegetation monitoring. Affinity selection of target cells from cell surface displayed libraries : a novel procedure using thermo-responsive magnetic nanoparticles. These results suggested that the Δ tubB strain was released from CCR. Effect of flocculation on performance of arming yeast in direct ethanol fermentation. Journal of the Japan Society of Photogrammetry and Remote Sensing, 39 5 , 23-34. Construction of a xylose-metabolizing yeast by genome integration of xylose isomerase gene and investigation of the effect of xylitol on fermentation. Engineering proteins to enhance their partition coefficients in aqueous two-phase systems. Physicochemical and immunological characterization of hepatitis B virus envelope particles exclusively consisting of the entire L Pre-S1+Pre-S2+S protein. Sortase A-Assisted Metabolic Enzyme Ligation in Escherichia coli for Enhancing Metabolic Flux. Alcoholic fermentation of xylose and mixed sugars using recombinant Saccharomyces cerevisiae engineered for xylose utilization. Development of efficient protein refolding systems using chaperonins. Energy-saving direct ethanol production from low-temperature-cooked corn starch using a cell-surface engineered yeast strain co-displaying glucoamylase and α-amylase. Adsorption of g-globulin, a model protein for antibody, on colloidal particles. Our study reveals the in vitro bifidogenic effect of DKT that likely contributes to its beneficial effects on the human colon. Water activity dependence of performance of surface-displayed lipase in yeast cells: A unique water requirement for enzymatic synthetic reaction in organic media. Environmental Geology, 44 5 , 564-571. Specific Protein Delivery to Target Cells by Antibody-Displaying Bionanocapsules. Circular dichroism studies on conformational changes in protein molecules upon adsorption on ultrafine polystyrene particles. Biotinylated bionanocapsules for displaying diverse ligands toward cell-specific delivery. Moreover, cellobiose transporter genes and the other sugar transporter genes were highly upregulated, and simultaneous uptake of glucose and cellobiose was also observed in the Δ tubB strain. Regulation of the display ratio of enzymes on the Saccharomyces cerevisiae cell surface by the immunoglobulin G and cellulosomal enzyme binding domains. Efficient production of ethanol from raw starch by a mated diploid Saccharomyces cerevisiae with integrated α-amylase and glucoamylase genes. Mathematical Problems in Engineering, Vol. Expression, crystallization and preliminary X-ray diffraction studies of thermostable β-1,3-xylanase from Thermotoga neapolitana strain DSM4359.。

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